Screening of zinc solubilizing bacteria and their potential to. Zinc solubilizing bacteria solubilize both the insoluble zinc compounds, though zno is more effectively solubilized in comparison to znco3 1. Effect of zinc solubilizing bacteria on growth promotion and. Isolation of zinc solubilizing bacteria from collected soil samples serial dilution pour plate technique was used for isolation of zinc solubilizing bacteria and 1 gm of rhizosphere soil from each sample was used for serial dilution. Indian conditions are tend to be deficient in zinc due to adverse climatic conditions, high ph and several other factors, whereas. Effect of zn enriched organic manures and zinc solubilizer. Zinc solubilising bacteria are potential alternates for zinc supplement. Keeping in view the above stated problem, the present study w as conducted. Among 10 strains screened for zn solubilisation, p29, p33, and b40 produced 22. Production and implication of bioactivated organic fertilizer. Expression of zinc transporter genes in rice as influenced. The plant growth promotory properties such as zn solubilization and iaa production of the isolates was checked in a previous study. Airborne bacteria isolated from a tannery air environment were screened for the property of solubilization of insoluble zinc oxide and zinc phosphate. Mishra ig, sapre s, tiwari s 2017 zinc solubilizing bacteria from the rhizosphere of rice as prospective modulator of zinc biofortification in rice.
Contribution of zinc solubilizing bacteria in growth promotion and. Agronomy free fulltext production and implication of. Attachment of soil bacteria to plant cells is supposedly the very early step required in plantmicrobe interactions. Microbes are potential alternate that could cater plant zinc requirement by solubilising the complex zinc in soil. For the production of zn solubilizing bacteria enriched bioorganic fertilizers boz, the powdered zinc oxide zno was mixed into the inoculated. Serial dilution pour plate technique was used for isolation of zinc solubilizing bacteria and 1gm of rhizosphere soil from each sample was used for. Zinc zn deficiency is well known problem occurring in both plants and humans. Nagalakshmi department of microbiology, kanchi shri krishna college of arts and science.
Introduction phosphorus p, after nitrogen is the major plant growthlimiting nutrient despite being. Shabnam s shaikh and meenu s saraf department of microbiology and biotechnology, school of. Research articles zinc solubilizing bacterial isolates. Zinc solubilizing bacteria for zinc biofortification in cereals. The application of zn solubilizing bacteria zsb increases the availability of native zinc for plant assimilation and eventually plant growth promotion. Pdf zinc solubilizing bacteria for zinc biofortification in cereals.
They are grampositive plant associated bacteria that possess strong growth promoting activities like solubilization of minerals, nitrogen fixation, production of. Effect of zn solubilizing bacteria on growth promotion and zn nutrition of rice 891 high cost associated with the application of zn fertilizers in order to correct zn deficiency places considerable burden on. Frontiers contribution of zinc solubilizing bacteria in. Isolation of siderophoreproducing and zincsolubilizing bacteria. Isolation and characterization of phosphate solubilizing microbes from agricultural soil t. Pdf screening and isolation of zinc solubilizing bacteria. Zinc solubilizing bacteria from rhizospheric soil of. The aim of this study was to isolate, characterize and use phosphate solubilizing bacteria to enhance the bioavailability of insoluble caphosphate for wheat plants. Addition of different insoluble sources of zinc zno, znco 3 and zn po 3 4 at 0. Soil and plant inoculation with heterotrophic zincsolubilizing bacteria zsb is considered a promising approach for increasing zinc zn phytoavailability and enhancing crop growth and. In this study, isolates were obtained from waste mushroom residues, which were composed of cotton.
In this study, three zn solubilising bacteria previously isolated from the rhizosphere of rice plants growing in zn deficient soil were evaluated singly as well as in. Effect of zinc solubilizing bacteria on growth promotion. Potassium solubilizing bacteria are capable of solubilizing rock k, mineral powder such as mica, illite and orthoclases through production and excretion of organic acids fridrich et al. The samples were serially diluted upto 105 dilution factor. Isolation, screening and characterization of phosphate. Zinc solubilizing bacteria solubilize both the insoluble zinc compounds, though zno is more effectively solubilized in comparison to. Contrarily, zinc oxide is a cheaper and insoluble source which contains 80% zn. Effects of root colonization by zincsolubilizing bacteria. Attachment also is an initial step for the formation of microbial biofilms on plant roots. When its availability drops below the critical level 2. Its deficiency in plants retards photosynthesis and nitrogen metabolism, causes the reduction in. Research article prospective zinc solubilising bacteria. This study found bacteria that solubilized phosphate at very high rates. Isolation of zinc solubilizing bacteria from soil samples.
Prospective zinc solubilising bacteria for enhanced. Occurrence, mechanisms and their role in crop production ahmad ali khan 1, ghulam jilani 1, mohammad saleem akhtar 1, syed muhammad saqlan naqvi 2, mohammad. Zinc zn is an element with a bipartite influence on living systems. F root tips, with white arrows showing the location of zincsolubilizing isolates. Production and implication of bioactivated organic. Inoculation of zincsolubilizing bacteria with different zinc. Pdf effect of zinc solubilizing bacteria on growth. Isolation and screening of potassium and zinc solubilizing. However, zinc solubilizing bacteria could be used to solubilize. Inorganic zinc in soil is generally in unavailable form for plant assimilation. Phosphate solubilizing bacteria psb have the potential to enhance phosphateinduced immobilization of metals to remediate contaminated soil. Phosphate solubilizing bacteria with glucose dehydrogenase. However, zinc solubilizing bacteria co uld be used to solubilize insoluble sources of zinc zno. Optimization of growth conditions for zinc solubilizing.
Zinc zn is among the essential micronutrients required for optimum plant growth. Bioactivated organic fertilizers boz were produced by enriching the zinc oxide znoorange peel waste composite with zn solubilizing bacteria zsb. A step toward sustainable nutritional security chapter pdf available june 2018 with 1,976 reads how we measure reads. The present study was designed to isolate and characterize zinc solubilizing bacteria zsb from the rhizosphere of rice and subsequent evaluation of these isolates for plant growth promotion of. However, the reports of zinc solubilizing bacteria in mangroves are largely missing, and hence the present study was made to isolate and enumerate the zinc solubilizing bacteria. Optimization of growth conditions forzinc solubilizing. Nutrients mobilization from zinc solubilizing bacteria is considered the most criti cal function to improve zinc bioavailability in soil for the plants. Optimization of growth conditions for zinc solubilizing plant growth associated bacteria and fungi. Isolation of zinc solubilizing bacteria from the rhizosphere by soil serial dilution plate method on mineral salts agar medium was amended with 0. Plant growth promotion of maize with zinc solubilizing pseudomonas sp.
Solubilization of inorganic mineral nutrients such as insoluble form zinc into accessible form for plant assimilation is a well known plant growth promoting traits exhibited by many bacteria. Solubilization of zinc salts by a bacterium isolated from. The maximum zinc solubilization zone was observed in isolate msszf1 90 mm, followed by msszf3 89 mm, msszf2 79 mm, msszb1 57 mm msszb2 45 mm msszb3 45 mm and msszb4. Eightyfive different soil samples were collected from the rhizosphere of different crops such as corn, sunflower, grapes, pistachio, alfalfa and. Pdf prospects of zinc solubilizing bacteria for enhancing growth of.
Substantial quantity of applied inorganic zinc in soil is converted into unavailable form. Isolation and characterization of phosphate solubilizing. Microbial zinc solubilization and their role on plants. For the production of zn solubilizing bacteria enriched bioorganic fertilizers boz, the. However, there is a limit on the amount of phosphate which. Insp6 induced low nutrient uptake poses a major public health problem in the form of zinc and iron deficiency, in populations that live mainly on grains and legumes. This study was conducted to screen zinc solubilizing rhizobacteria isolated from wheat and sugarcane, and to analyze their effect on wheat growth and development. Zinc solubilizing bacteria from rhizospheric soil of mangroves. Zinc zn is one of the essential micronutrients required for optimum plant growth.
Several bacteria associated with roots, have the ability to increase plant growth and productivity by increasing the supply of mineral nutrients of low mobility in the soil like zinc and it is called zinc. Pikovskayas agar was modified by sundara rao and sinha 2 for detection of phosphatesolubilizing bacteria from soil. The zinc tolerance limit for two cultures zsbo1 and zsbs2 was studied and determined to be upto 100 mg kg1 of zinc in the in vitro broth assay. However, only some bacterial species of the genera acinetobacter, bacillus, gluconacetobacter, and pseudomonas have been reported to solubilize zinc. Pdf zinc solubilizing bacteria for zinc biofortification.
The binding can result in very insoluble salts with poor bioavailability of minerals rhou and erdman, 1995. Zinc is an imperative micronutrient required for optimum plant growth. Isolation of efficient phosphate solubilizing bacteria. In a recent decade, importance of zn solubilizing bacteria zsb has. Pdf biofilm formation of zinc solubilizing, potassium. Zinc solubilizing microorganisms solubilize zinc through various mechanisms, one of which is acidification. In the current study, seed bacterization with zinc solubilizing plant growth promoting bacteria resulted in increased plant height root volume and shoot height. Enumeration and isolation of zinc solubilizing bacteria enumeration of zinc solubilizing bacteria zsb present in the soil samples was done by adopting plate count method. Scanning electron microscopy micrographs showing the location of zincsolubilizing bacteria colonization inoculated on mr219 rice roots. Zinc solubilizing bacteria from the rhizosphere of rice as.
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